Xiaomei Yan, Ph.D.

 

Laboratory Address                                            Home Address

Department of Chemical Biology

College of Chemistry and Chemical Engineering                       Daxuecheng B-1501

Xiamen University                                                     Yanwu Rd, Siming District

Xiamen, Fujian 361005, China                                  Xiamen, Fujian 361005, China

Office: (86) 0592-2184519                                        Phone: (86)0592-2184576

Education

Xiamen University,                                           Ph.D. 1996  Analytical Chemistry

      Xiamen, Fujian, China

Xiamen University,                                B.E.   1990  Analytical Instrumentation

Professional Experience

Professor, 2005 - present

                 College of Chemistry and Chemical Engineering, Xiamen University, China

Technical Staff Member, National Flow Cytometry Resource, 2001 - 2005

                 Bioscience Division, Los Alamos National Laboratory, New Mexico, USA

Post-doctoral researcher, 1997 - 2001

                 Bioscience Division, Los Alamos National Laboratory, New Mexico, USA

Post-doctoral researcher, 1996 - 1997

                 Department of Chemistry, University of Florida, Gainesville, Florida, USA.

Honors, Awards

The 12th Youth Science and Technology Award of Fujian Province, 2014

National Young and Middle-aged Leading Talents in Science and Technology Innovation, 2013, Ministry of Science and Technology

May 1 labor medals, Xiamen City, 2013

Leading Scientist of Fujian Province Universities, 2013

National Science Fund for Distinguished Young Scholars of China, 2012

New Century Excellent Talents in University (NCET) by MOE of China, 2007

Strategic hire fund ($100K), Los Alamos National Laboratory, USA, 2002

Bioscience Division Employee Award, Los Alamos National Laboratory, USA, 2001

Japanese Government Scholarship, Japan, 1994 - 1996

Outstanding Graduate Student Award, Xiamen University, 1993

First-grade Graduate Student Research Achievement Award, Xiamen University, 1992

Recent Fundings

Principal Investigator: NSFC 91313302, "Development of New Analytical Methodologies for Signal Transduction Pathway Study" (2014-2015), ~$200,000.

 

Principal Investigator: NSFC 21225523, "Biochemical Analysis and Biosensing" (2013-2016), National Science Fund for Distinguished Young Scholars, ~$330,000.

 

Principal Investigator: NSFC 21027010, "Development of High Sensitivity Flow Cytometry and the Nano-bio Characterization System"  (2011-2013), ~$300,000.

 

Principal Investigator: NSFC 90913015, "Study Mitochondria-Mediated Apoptotic Signal Transduction and the Activation Mechanisms of Anticancer Drugs by High Sensitivity Flow Cytometry" (2010-2012), ~$80,000.

 

Principal Investigator: NSFC 20975087, "Development and Applications of High Sensitivity Flow Cytometry in the Rapid and Sensitive Detection of Bacteria" (2010-2012), ~$55,000 total costs.

 

Principal Investigator: NCET-07-0729, "Biochemical Analysis and Biosensing" (2008-2010), New Century Excellent Talents in University (NCET) by MOE of China, ~$80,000 total costs.

 

Principal Investigator: NSFC 20375070, "Single Molecule Detection in Flow and Ultrasensitive Immuno-Analysis of Protein Markers" (2007-2009), ~$45,000 total costs.

 

Research Interests

Development of advanced instrumentation and methodologies for biochemical analysis and biosensing. The main research focuses include：

 

1) Development of high sensitivity flow cytometry (HSFCM) to address the challenge of quantitative physical and biochemical characterization of single nanoparticles in nanotechnology, nanobiotechnology, nanomedicine, biopharmaceuticals, and basic science.

2) Specific analysis of individual bacteria for pathogen identification, antibiotic resistance detection, and biological function studies.

3) Development of advanced methodologies for the individual analysis of subcellular organelles such as mitochondria, extracellular vesicles (microvesicles and exosomes), and chromosomes.

4) High-throughput, high-resolution, and multiparameter characterization of single natural and synthetic nanoparticles such as gold, silver, silica, polymeric, and iron oxide nanoparticles, lipid-based nanomedicines, and quantum dots.

5) Development of microsphere-based suspension array technology for biomedical and biochemical applications.

 

Publications

1. Yu, MX; Wu, LN; Huang, TX; Wang, S; Yan, XM*. Rapid detection and enumeration of total bacteria in drinking water and tea beverages by a laboratory-built high-sensitivity flow cytometer, Analytical Methods, 2015, 7, 3072-3079.
2. Zhang, Z*; Deng, CQ; Meng, LS; Zheng, Y; Yan, XM. A rhodamine hydrazide-based fluorescent probe for sensitive and selective detection of hypochlorous acid and its application in living cells, Analytical Methods, 2015, 7, 107-114.
3. Zhu, SB; Ma, L; Wang, S; Chen, CX; Zhang, WQ; Yang, LL; Hang, W; Nolan, J. P.; Wu, LN; Yan, XM*. Light-scattering detection below the level of single fluorescent molecules for high-resolution characterization of functional nanoparticles. ACS Nano 2014, 8, 10998-11006.
4. Zhang, X; Zhang, SY; Zhu, SB; Chen, S; Han, JY; Gao, KM; Zeng, JZ; Yan, XM*. Rapid identification of mitochondria-targeting anticancer compounds by an in vitro strategy, Analytical Chemistry, 2014, 86, 5232−5237.
5. Wu, LN; Luan, T; Yang, XT; Wang, S; Zheng, Y; Huang, TX; Zhu, SB; Yan, XM*. Trace detection of specific viable bacteria using tetracysteine-tagged bacteriophages, Analytical Chemistry, 2014, 86, 907-912.
6. Yang, CW; Chen, S; Zhou, M; Li, Y; Li, YF; Zhang, ZX; Liu, Z; Ba, Q; Li, JQ; Wang, H; Yan, XM*; Ma, DW*; Wang, RX*. Development of 3-Phenyl-N-(2-(3-phenylureido) ethyl)-thiophene-2-sulfonamide compounds as inhibitors of antiapoptotic Bcl-2 family proteins, ChemMedChem, 2014, 9, 1436 - 1452.
7. Xu, F; Zhu, L; Zhu, SB; Yan, XM; Xu, HC*. Electrochemical intramolecular aminooxygenation of unactivated alkenes, Chemistry-A European Journal, 2014, 20, 12740- 12744.
8. Huang, ZZ; Zhang, SD; Hang, W*; Chen, YD; Zheng, JX; Li, W; Xing, JC; Zhu, EY; Yan, XM. Liquid chromatography-mass spectrometry based serum peptidomic approach for renal clear cell carcinoma diagnosis, Journal of Pharmaceutical and Biomedical Analysis, 2014, 100, 175-183.
9. Shao, Q; Zheng, Y; Dong, XM; Tang, K; Yan, XM*; Xing, BG*. A covalent reporter of b-lactamase activity for fluorescent imaging and rapid screening of antibiotic-resistant bacteria, Chemistry-A European Journal, 2013, 19, 10903-10910.
10. Yang, LL; Huang, TX; Zhu, SB; Zhou YX; Jiang, YB; Wang, S; Chen, YQ; Wu, LN; Yan XM*. High-throughput single-cell analysis of low copy number b-Galactosidase by a laboratory-built high-sensitivity flow cytometer, Biosensors & Bioelectronics, 2013, 48, 49-56.
11. Chen, CX; Zhu SB; Huang TX; Wang S; Yan, XM*. Analytical techniques for single-liposome characterization. Analytical Methods, 2013, 5, 2150-2157.
12. Yang, L; Wu, SQ; Lin, BJ; Huang, TX; Chen, XP; Yan, XM; Han, SF*. A targetable nanogenerator of nitric oxide for light-triggered cytotoxicity. Journal of Material Chemistry, B, 2013, 1, 6115.
13. Huang, ZZ; Chen, YJ; Hang, W.*, Gao, Y.; Lin, L.; Li, D. Y.; Xing, JC; Yan, XM. Holistic Metabonomic Profiling of Urine Affords Potential Early Diagnosis for Bladder and Kidney Cancers, Metabolomics, 2013, 9, 119-129.
14. Zhang, SY; Zhu, SB; Yang, LL; Zheng, Y; Gao, M; Wang, S; Zeng, J-Z; and Yan, XM*. High-throughput multiparameter analysis of individual mitochondria, Analytical Chemistry, 2012, 84, 6421-6428.
15. Yang, LL; Zhou, YX; Zhu, SB; Huang, TX; Wu, LN; Yan, XM*. Detection and quantification of bacterial autofluorescence at the single-cell level by a laboratory-built high-sensitivity flow cytometer, Analytical Chemistry, 2012, 84, 1526-1532.
16. Huang, H; Chen, ZJ; Yan, XM*. Simultaneous determination of serotonin and creatinine in urine by combining two ultrasound-assisted emulsification microextractions with on-column stacking in capillary electrophoresis, Journal of Separation Science, 2012, 35, 436-444.
17. Lin, L; Huang, ZZ; Gao, Y; Chen, YJ; Hang, W.*, Xing, JC; Yan, XM. LC-MS based serum metabolic profiling for genitourinary cancer classification and cancer type-specific biomarker discovery, Proteomics, 2012, 12, 2238-2246.
18. Lu, N.; Liu, JX; Liu, J.; Zhang, CY; Jiang, FQ; Wu, H.; Chen, LQ; Zeng, WJ; Cao, XH; Yan, TD; Wang, GH; Zhou, H.; Lin, BZ; Yan, XM; Zhang, XK; and Zeng, JZ*. Antagonist effect of triptolide on AKT activation by truncated retinoid X receptor-alpha. PLoS One, 2012, 7(4): e35722.
19. Gao, Y.; Yang, N.; Yan, XM; Hang, W.*; Xing, JC; Zheng, J.; Zhu, EY; Huang, BL. Early diagnosis of urinary lithiasis via elementary profile of serum samples, Analytical Methods, 2012, 4, 693-698.
20. Wu, LN; Huang, TT; Yang, LL; Pan, JB; Zhu, SB; Yan, XM*. Sensitive and selective bacterial detection using tetracysteine-tagged phages in conjunction with biarsenical dye, Angewandte Chemie International Edition, 50, 5873-5877.
21. Zhu, SB; Wang, S; Yang, LL; Huang, TX; Yan, XM*. Progress in the development of techniques based on light scattering for single nanoparticle detection, Science China Chemistry, 2011, 54, 1244-1253.
22. Zheng, Y; Lin, L; Hang, W; Yan, XM*; Marrone, B. L.* Analysis of beryllium to biomolecule binding using a metal specific fluorescent probe and competitive Assay, Talanta, 2011, 85, 638-643. 
23. Zhang, Z*; Zheng, Y; Hang, W; Yan, XM*; Zhao, YF. Sensitive and selective off-on rhodamine hydrazide fluorescent chemosensor for hypochlorous acid detection and bioimaging, Talanta, 2011, 85, 779-786.
24. Huang, ZZ; Lin, L; Gao, Y; Chen, YJ; Yan, XM; Xing, JC; Hang, W*. Bladder cancer determination via two urinary metabolites: a biomarker pattern approach, Mol. Cell. Proteomics, 2011, 10, 10.1074/mcp.M111.007922-1–007922-10.
25. Lin, L; Huang, ZZ; Gao, Y; Yan, XM; Xing, JC; Hang, W*. LC-MS based serum metabonomic analysis for renal cell carcinoma diagnosis, staging, and biomarker discovery, Journal of Proteome Research, 2011, 10, 1396-1405.
26. Zhu, SB; Yang, LL; Long, Y; Gao, M; Huang, TX; Hang, W; Yan, XM*. Size differentiation and absolute quantification of gold nanoparticles via single particle detection with a laboratory-built high-sensitivity flow Cytometer, Journal of  the American Chemical Society, 2010, 132, 12176-12178.
27. Yang, LL; Wu, LN; Zhu, SB; Long, Y; Hang, W; Yan, XM*. Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow Cytometer, Analytical Chemistry, 2010, 82, 1109-1116.
28. Zhang, ZL; Long, Y; Pan, JB; Yan, XM*. Preparation of fluorescence-encoded microspheres in a core-shell structure for suspension arrays, Journal of Material Chemistry, 2010, 20, 1179-1185.
29. Long, Y; Zhang, ZL; Yan, XM*; Xing, JC*; Zhang, KY; Huang, JX; Zheng, JX; Li, W. Multiplex immunodetection of tumor markers with a suspension array built upon core-shell structured functional fluorescence-encoded microspheres, Analytica Chimica Acta, 2010, 665, 63-68.
30. Yan, XM; Lin, YM; Huang, RF; Hang, W*; Harrison, W. W. A spectroscopic investigation of the afterglow and recombination process in a microsecond pulsed glow discharge, Journal of Analytical Atomic Spectrometry, 2010, 25, 534-543.
31. Lin, L; Yu, Q; Yan, XM; Hang, W*; Zheng, JX; Xing, JC; Huang, BL. Direct infusion mass spectrometry or liquid chromatography mass spectrometry for human metabonomics? A serum metabonomic study of kidney cancer, Analyst, 2010, 135, 2970-2978.
32. Xing, JC*; Yan, LJ; Zhang, J*; Lin, L; Gao, Y; Chen, WG; Song, XY; Yan, XM; Hang, W*; Huang, BL. A comparative study of elution gradients in UPLC-TOF-MS-based metabonomics research, Chromatographia, 2010, 72, 807-813.
33. Yang, LL; Zhu, SB; Hang, W; Wu, LN; Yan, XM*. Development of an ultrasensitive dual-channel flow cytometer for the individual analysis of nanosized particles and biomolecules, Analytical Chemistry, 2009, 81, 2555-2563.
34. Ding, J; Lin, L; Hang, W; Yan, XM*. Beryllium uptake and related biological effects studied in THP-1 differentiated macrophages, Metallomics, 2009, 1, 471-478.
35. Chen, ZJ; Yan, XM*. Simultaneous determination of melamine and 5-hydroxymethylfurfural in milk by capillary electrophoresis with diode array detection, Journal of agricultural and food chemistry, 2009, 57, 8742-8747.
36. Zhang, J*; Yan, LJ; Chen, WG; Lin, L; Song, XY; Yan, XM; Hang, W*; Huang, BL. Metabonomics research of diabetic nephropathy and type 2 diabetes mellitus based on UPLC-oaTOF-MS system, Analytica Chimica Acta, 2009, 650, 16-22.
37. Chen, LZ; Lin, L; Yu, Q; Yan, XM; Hang, W*; He, J; Huang, BL. Semiquantitative multielemental analysis of biological samples by a laser ionization orthogonal time-of-flight mass spectrometer, Journal of the American Society for Mass Spectrometry, 2009, 20, 1355-1358.
38. Yang, LL; Zhu, SB; Hang, W; Yan, XM*. Development of a single-molecule flow analyzer, Chemical Journal of Chinese Universities-Chinese, 2008, 29, 1549-1551.
39. Zhang, J*; Yan, LJ; Lin, L; Chen, WG; Song, XY; Yan, XM; Hang, W*; Huang, BL. Metabonomics research of diabetic mellitus and diabetic nephropathy based on UPLC-oaTOF-MS system, Chemical Journal of Chinese Universities-Chinese, 2008, 29, 2171-2173.
40. He, J; Zhong, WW; Tang, AJ; Yan, XM; Lewis, C.; Majidi, V.; Hang, W. A fluorescence detection scheme for ultra large molecules after gas phase separation, Talanta, 2007, 71, 2126-2128. 
41. Yan, XM*; Zhong, WW; Tang, AJ; Schielke, E. G.; Hang W, Nolan J. P. Multiplexed flow cytometric immunoassay for influenza virus detection and differentiation, Analytical Chemistry, 2005, 77, 7673-7678. 
42. Yan, XM*; Habbersett R. C.; Yoshida, T. M.; Nolan, J. P.; Jett, J. H.; Marrone, B. L. Probing the kinetics of SYTOX Orange stain binding to double-stranded DNA with implications for DNA analysis, Analytical Chemistry, 2005, 77, 3554-3562.
43. Yan, XM*; Tang, AJ; Schielke, E.G.; Hang, W; Nolan, J. P. On the development of a microsphere-based multiplexed immunoassay for influenza virus typing and subtyping by flow cytometry, Options for the control of influenza V, International Congress Series, 2004, 1263, 342-345.
44. Yan, XM*; Schielke, E.G.; Grace, K.M.; Hassell, C.; Marrone, B. L.; Nolan, J. P.* Microsphere-based duplexed immunoassay for influenza virus typing by flow cytometry, Journal of Immunological Methods, 2004, 284, 27-38.
45. Ferris, M. M.; Yan, XM; Habbersett, R. C.; Shou, YL; Lemanski, C. L.; Jett, J. H.; Yoshida, T. M.; Marrone, B. L. Performance assessment of DNA fragment sizing by high sensitivity flow cytometry and pulsed field gel electrophoresis, Journal of Clinical Microbiology, 2004, 42, 1965-1976.
46. Yan, XM; Hang, W; Majidi, V.; Marrone, B. L.; Yoshida, T. M. Evaluation of different nucleic acid stains for sensitive dsDNA analysis with capillary electrophoresis separation, Journal of Chromatography A, 2002, 943, 275-285. 
47. Yan, XM; Ingeneri, K.; Hang, W; Harrison, W. W. Factors influencing signal profiles in microsecond pulsed glow discharge atomic emission spectrometry, Journal of Analytical Atomic Spectrometry, 2001, 16, 819-824.
48. Yan, XM; Habbersett, R. C.; Cordek, J. M.; Nolan, J. P.; Yoshida, T. M.; Jett, J. H.; Marrone, B. L. Development of a mechanism-based, DNA staining protocol using SYTOX Orange nucleic acid stain and DNA fragment sizing flow cytometry, Analytical Biochemistry, 2000, 286, 138-148.
49. Yan, XM; Grace, W. K.; Yoshida, T. M.; Habbersett, R. C.; Velapan, N.; Jett, J. H.; Keller, R. A.; Marrone, B. L. Characteristics of different nucleic acid staining dyes for DNA fragment sizing by flow cytometry, Analytical Chemistry, 1999, 71, 5470-5480.
50. Hang, W; Yan, XM; Wayne, D. M.*; Olivares, J. A.; Harrison, W. W.; Majidi, V. Glow discharge source interfacing to mass analyzers: Theoretical and practical considerations, Analytical Chemistry, 1999, 71, 3231-3237.
51. Hang, W; Yan, XM; Wayne, D. M.; Olivares, J. A.; Harrison, W. W.; Majidi, V.* Selective removal of matrix ion peaks in plasma source time-of-flight mass spectrometry: ion deflection and detector gating, Journal of Analytical Atomic Spectrometry, 1999, 14, 1523-1526.
52. Duan, YX; Danen, R. E.; Yan, XM; Steiner, R.; Cuadrado, J.; Wayne, D.; Majidi, V.; Olivares, J. A*. Characterization of an improved thermal ionization cavity source for mass spectrometry, Journal of the American Society for Mass Spectrometry, 1999, 10, 1008-1015.
53. Yan, XM; Hang, W; Smith, B. W.; Winefordner, J. D.; Harrison, W. W.* Primary studied of microsecond pulsed glow discharge as an emission sources by using conventional hollow cathode lamp, Journal of Analytical Atomic Spectrometry, 1998, 13, 1033-1038.
54. Harrison, W. W.*; Hang, W; Yan, XM; Ingeneri, K.; Schilling, C. Temporal considerations with a pulsed glow discharge”, Journal of Analytical Atomic Spectrometry, 1997, 12, 891-896.
55. Yan, XM*; Huang, BL; Tanaka, T.; Kawaguchi, H. Langmuir probe potential measurements for reduced pressure inductively coupled plasma mass spectrometry, Journal of Analytical Atomic Spectrometry, 1997, 12, 697-701.
56. Yan, XM; Tanaka, T.; Kawaguchi, H.* Electrothermal vaporization for the determination of halogens by reduced pressure inductively coupled plasma mass spectrometry, Spectrochimica Acta Part B, 1996, 51, 1345-1353.
57. Yan, XM; Tanaka, T.; Kawaguchi, H.* Reduced pressure inductively coupled plasma mass spectrometry for nonmetallic elements, Applied Spectroscopy, 1996, 50, 182-187.
58. Wang, XR*; Zhuang, ZX; Yang, CL; Yang, PY; Yan, XM; Lin, JM. Development of transient data acquisition system for hyphenated techniques coupled with inductively coupled plasma atomic emission spectrometry, Spectrochimica Acta Part B, 1996, 51, 1753-1759.
59. Yan, XM; Wang, XR; Yang, PY; Hang, W; Huang, BL*. Signal Acquisition and processing in transient introduction techniques on-line hyphenated with multichannel ICP-AES, Chemical Journal of Chinese Universities-Chinese, 1993, 14, 1506-1509.
60. Wang, XR*; Zhu, EY; Yan, XM; Yang, PY; Huang, BL; Zhuang, ZX. Chemometrics study on preliminary clinical diagnostics for cancer patients, Acta Chimica Sinica, 1993, 51, 1094-1098.